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(NIH) In adults over 55 with type-2 diabetes, GSH supplementation improved glutathione stores and reduced DNA oxidative damage (8-OHdG) while lowering HbA1c.(NIH) However, short-term trials (4 weeks) in healthy adults showed no measurable change in blood GSH, highlighting absorption variability
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Human observational or clinical evidence was considered more directly translatable, whereas animal, cell-based, species-specific sperm preservation, and in silico studies were regarded as preclinical or hypothesis-generating evidence
Gene set enrichment analysis (GSEA) confirmed the association between GSH metabolism and MTX resistance
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